Is the association between the severity of airflow limitation and DNA Methylation similar in blood and lung tissue in patients with COPD?
Background: Epigenetic Changes and DNA Methylation in COPD
Previous studies have reported epigenetic changes in lung tissue or blood of COPD patients but whether the relationship of methylation changes with the severity of airflow limitation is similar or different in both tissues is unknown.
Methods: Assessing DNA Methylation in Blood and Lung Tissue of COPD Patients
DNA was extracted from blood (n=269) and lung tissue (n=140) of COPD patients with different levels of airflow limitation (FEV1 % ref.). DNA methylation was assessed with the EPIC array (Illumina).
CpG-sites associated with FEV1 %ref. in blood and lung tissue, were determined using multivariate regression adjusted for age, sex, smoking status and packs/year.
Results: Opposite Associations of DNA Methylation with Airflow Limitation in COPD
Both in blood and lung tissue, 4,979 CpGs were associated with FEV1% ref. (FDR<0.05). Interestingly, 98% of these CpGs presented an opposite association with FEV1% ref. between the two tissues.
Annotated genes showing a negative association with FEV1% ref. in blood, but a positive association in lung tissue were enriched for ontologies related to actin organization, T cell differentiation, cell signaling and morphogenesis.
Conversely, annotated genes with a positive association to FEV1% ref. in blood (but negative in lung tissue) were enriched for cell junction assembly, cell-matrix adhesion and cell migration ontologies.
Conclusions: Differential Regulation of DNA Methylation in Blood and Lung Tissues in COPD
Blood and lung DNA share differentially methylated positions in relation to the severity of airflow limitation.
However most of them present an opposite direction of effect suggesting that both tissues are differentially regulated.
Authors
Sandra Casas Recasens, Nuria Mendoza, Nuria Olvera, Lidia Perea, Tamara Garcia, Oriol Sibila, Àlvar Agustí, Rosa Faner
Read more details at
Noticias relacionadas
Relationship between Respiratory Microbiome and Systemic Inflammatory Markers in COPD: A Pilot Study
This study explores how changes in the respiratory microbiome relate to systemic inflammation in COPD patients. It highlights correlations between bacterial abundance, eosinophilic markers, and airflow limitation severity.
Investigación Médica en Hospitales: Conferencia inaugural de las sesiones de médicos residentes del hospital Clinic de Barcelona.
¿Por qué la investigar en un hospital? Porque la investigación médica en hospitales es la ÚNICA forma en la que la medicina puede avanzar.
Monitoring and assessment of CO2 and NO2 in schools within the Sentinel Schools Network of Catalonia during the COVID-19 era
Explore how the COVID-19 Sentinel Schools Network of Catalonia monitored CO2 and NO2 levels in classrooms, revealing key findings on ventilation and air quality in schools across Catalonia during the pandemic.
Artículos
COPD
- 759578·Alberto Papi et Al.-Relationships between symptoms and lung function in asthma and/or chronic obstructive pulmonary disease in a real-life setting: the NOVEL observational longiTudinal studY
- 759785·Richard Beasley et Al – Prevalence, Diagnostic Utility and Associated Characteristics of Bronchodilator Responsiveness
- 759788·Alvar Agustí, Rod Hughes, Eleni Rapsomaki, Barry Make, Ricardo Del Olmo, Alberto Papi, David Price, Laura Benton, Stefan Franzen, Jørgen Vestbo, Hana Mullerova – The many faces of COPD in real life: a longitudinal analysis of the NOVELTY cohort
- 759883·Alberto Papi, Rosa Faner, Ian Pavord, Federico Baraldi, Vanessa M McDonald, Mike Thomas, Marc Miravitlles, Nicholas Roche, Alvar Agustí. – From treatable traits to GETomics in airway disease: moving towards clinical practice
- 768799·Surya P Bhatt Richard Casaburi Alvar Agusti et Al. Chronic obstructive pulmonary disease: hiding in plain sight, a Statement from the COPD Foundation Medical and Scientific Advisory Committee
Imagen desarrollada desde DALL-E y editada en Canva
